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A Portable Lipid Bilayer System for Environmental Sensing with a Transmembrane Protein |
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| Authors: | Ryuji Kawano Yutaro Tsuji Koki Kamiya Taiga Kodama Toshihisa Osaki Norihisa Miki Shoji Takeuchi |
| Affiliation: | 1. Artificial Cell Membrane Systems Group, Kanagawa Academy of Science and Technology (KAST), Takatsu-ku, Kawasaki, Japan.; 2. Department of Mechanical Engineering, Keio University, Kohoku-ku, Yokohama, Japan.; 3. CIRMM-IIS, The University of Tokyo, Meguro-ku, Tokyo, Japan.; Texas A&M University, United States of America, |
| Abstract: | This paper describes a portable measurement system for current signals of an ion channel that is composed of a planar lipid bilayer. A stable and reproducible lipid bilayer is formed in outdoor environments by using a droplet contact method with a micropipette. Using this system, we demonstrated that the single-channel recording of a transmembrane protein (alpha-hemolysin) was achieved in the field at a high-altitude (∼3623 m). This system would be broadly applicable for obtaining environmental measurements using membrane proteins as a highly sensitive sensor. |
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