Molecular mapping of the Oregon Wolfe Barleys: a phenotypically polymorphic doubled-haploid population |
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| Authors: | J M Costa A Corey P M Hayes C Jobet A Kleinhofs A Kopisch-Obusch S F Kramer D Kudrna M Li O Riera-Lizarazu K Sato P Szucs T Toojinda MI Vales R I Wolfe |
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| Institution: | (1) Department of Natural Resource Sciences and Landscape Architecture, 2102 Plant Sciences Building, University of Maryland, College Park, MD 20742-4452, USA e-mail: jc274@umail.umd.edu, US;(2) Department of Crop and Soil Sciences, Oregon State University, Corvallis, OR 97331, USA, US;(3) INIA Carillanca, Temuco, Chile, CL;(4) Department of Soil and Crop Sciences, Washington State University, Pullman, WA 99164-6420, USA, US;(5) Institute of Genetics, Chinese Academy of Sciences, Beijing, China, CN;(6) Research Institute for Bioresources, Okayama University, Japan, JP;(7) Agricultural Research Institute of the Hungarian Academy of Sciences, H-2462 Martonvásár, Hungary, HU;(8) DNA Technology Laboratory, Kasetsart University, Nakorn Pathom 73140, Thailand, TH;(9) Agriculture and Agri-Food Canada, Field Crop Development Centre, 5030-50th Street, Lacombe, Alberta T4L 1W8, Canada, CA |
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| Abstract: | A phenotypically polymorphic barley (Hordeum vulgare L.) mapping population was developed using morphological marker stocks as parents. Ninety-four doubled-haploid lines were
derived for genetic mapping from an F1 using the Hordeum bulbosum system. A linkage map was constructed using 12 morphological markers, 87 restriction fragment length polymorphism (RFLP),
five random amplified polymorphic DNA (RAPD), one sequence-tagged site (STS), one intron fragment length polymorphism (IFLP),
33 simple sequence repeat (SSR), and 586 amplified fragment length polymorphism (AFLP) markers. The genetic map spanned 1,387
cM with an average density of one marker every 1.9 cM. AFLP markers tended to cluster on centromeric regions and were more
abundant on chromosome 1 (7H). RAPD markers showed a high level of segregation distortion, 54% compared with the 26% observed
for AFLP markers, 27% for SSR markers, and 18% for RFLP markers. Three major regions of segregation distortion, based on RFLP
and morphological markers, were located on chromosomes 2 (2H), 3 (3H), and 7 (5H). Segregation distortion may indicate that
preferential gametic selection occurred during the development of the doubled-haploid lines. This may be due to the extreme
phenotypes determined by alleles at morphological trait loci of the dominant and recessive parental stocks. Several molecular
markers were found to be closely linked to morphological loci. The linkage map reported herein will be useful in integrating
data on quantitative traits with morphological variants and should aid in map-based cloning of genes controlling morphological
traits.
Received: 23 August 2000 / Accepted: 15 December 2000 |
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| Keywords: | Barley Genetic map Molecular markers Segregation distortion Morphological markers |
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