Bleomycin-A2 complexes with poly(dA--dT): a proton nuclear magnetic resonance study of the nonexchangeable hydrogens |
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| Authors: | D M Chen T T Sakai J D Glickson D J Patel |
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| Affiliation: | 1. Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030 USA;2. Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX 77030 USA |
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| Abstract: | The mRNA coding for uteroglobin, a progesterone-induced uterine protein, has been partially purified from 4-day pregnant rabbit uterus. Double-stranded DNA synthesized from the partially purified mRNA preparation was inserted into the Pst I site of pBR 322. Bacterial transformants containing uteroglobin DNA sequences were identified by their ability to enrich for uteroglobin mRNA on hybridization with total uterine poly A-RNA. The identity of one recombinant was confirmed unambiguously by matching its nucleotide sequence with the amino acid sequence of the uteroglobin polypeptide. |
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| Keywords: | To whom all correspondence should be addressed. |
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