Lambda phage mutants insensitive to temperature-sensitive repressor |
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Authors: | Tadao Horichi Hideo Koga Hachiro Inokuchi and Jun-ichi Tomizawa |
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Institution: | (1) Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan;(2) National Institute of Health, Shinagawaku, Tokyo, Japan;(3) Present address: Faculty of Science, Osaka University, 560 Toyonaka, Osaka, Japan |
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Abstract: | Summary Weak-virulent mutants of temperate coli-phage were isolated which can grow on the CIts lysogen producing a temperature-sensitive repressor but which cannot grow on the wild type lysogen producing a normal repressor.Genetic analysis on the mutants shows that their weak-virulence is attributable to two mutations, one (virL) in the region between sus N213 and c
47 and the other (virR) in the region between c
1 and sus O8. Both mutations are located within the region of non-homology between and imm
434 phages.True virulent mutants which can grow on the wild type lysogen can be obtained easily from the weak-virulent mutant by an additional mutation, virC in a region very close to virR. The virulent mutants obtained are similar to the classical vir mutant (Jacob and Wollman, 1954). The virL and virR mutations are probably operator mutations which render the genome insensitive to the repressor.This work was reported at the XII th International Congress of Genetics, held in Tokyo, on August 23, 1968. |
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