Nephrotoxicity of Penicillium aurantiogriseum,a possible factor in the aetiology of Balkan Endemic Nephropathy |
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Authors: | S E Yeulet P G Mantle M S Rudge J B Greig |
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Institution: | (1) Department of Biochemistry, Imperial College, SW7 2AY London;(2) Medical Research Council Toxicology Unit, Carshalton, Surrey, UK |
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Abstract: | Water-soluble components of a nephrotoxic isolate of Penicillium aurantiogriseum have been fractionated by sequential ion-exchange, size-exclusion gel filtration, reverse-phase silica chromatography and HPLC. Nephrotoxicity in the rat was confined to a size-exclusion fraction approximating to 1500 daltons, which also inhibited DNA synthesis in cultured kidney cells. The more sensitive in vitro assay allowed toxicity to be followed to a sub-fraction from gradient-elution HPLC which in further HPLC resolved into a small group of glycopeptides. Recent Yugoslavian P. aurantiogriseum isolates, from a village in which the idiopathic human disease Balkan Nephropathy is hyperendemic, elicited a similar nephropathology and were acutely cytotoxic, reinforcing a need to regard this novel Penicillium nephrotoxin as a potential factor in human nephropathy. |
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Keywords: | nephrotoxin Penicillium aurantiogriseum Balkan Nephropathy mycotoxin glycopeptide cytotoxicity |
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