| 小鼠胸腺哺育细胞内淋巴细胞碱性磷酸酶电镜观察 |
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| 引用本文： | 毛曦,缪继武,尹兰珍.小鼠胸腺哺育细胞内淋巴细胞碱性磷酸酶电镜观察[J].中国组织化学与细胞化学杂志,1997(1). |
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| 作者姓名： | 毛曦 缪继武 尹兰珍 |
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| 作者单位： | 江苏职工医科大学，南京铁道医学院 |
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| 摘 要： | 碱性磷酸酶（ａｌｋａｌｉｎｅｐｈｏｓｐｈａｔａｓｅ，ＡＬＰ）是研究Ｔ淋巴细胞分裂分化的标记之一。胸腺内淋巴母细胞具有膜性ＡＬＰ。本文采用ＡＬＰ超微结构细胞化学方法，探讨小鼠胸腺哺育细胞（Ｔｈｙｍｉｃｎｕｒｓｅｃｅｌ，ＴＮＣ）内淋巴细胞（ＴＮＣ－Ｌ）ＡＬＰ的活性分布。观察结果，２０％的ＴＮＣ内有少数淋巴细胞呈阳性反应，而ＴＮＣ外游离的淋巴细胞均为ＡＬＰ阴性反应。结果表明，部分ＴＮＣ内含有少量淋巴母细胞亚群，ＴＮＣ是Ｔ淋巴细胞分裂分化的微环境之一
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| 关 键 词： | 胸腺哺育细胞 淋巴细胞 碱性磷酸酶 小鼠 电镜 |
ELECTRON MICROSCOPIC OBSERVATION OF ALKALINE PHOSPHATASE OF LYMPHOCYTE WITHIN THYMIC NURSE CELL (TNC) IN MICE |
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| Abstract: | Alkaline phosphatase(ALP) is one of the markers used to study the division and differentiation of T Lymphocyte. The lymphoblasts in thymus possess membrane ALPase activity. In our studies the distribution of ALPase activity of lymphocytes within thymic nurse cell(TNC-L) was investigated by means of an ultrastructural cytochemical method. The observation revealed that within 20% TNC-L a small number of lymphocytes showed ALPase-positive activity, however, the free lymphocytes outside TNC appeared as the negative reaction. The result indicates that there are a few subpopulations of lymphoblast in parts of TNC and TNC is one of the microenvironments for the Tlymphocyte division and differentiation. |
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| Keywords: | Thymic nurse cell Lymphocyte Alkaline phosphatase Mice Electron microscope |
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