Demonstration of a specific binding protein for testosterone and 5alpha-dihydrotestosterone in rabbit serum. Separation from the corticosteroid binding globulin (CBG) |
| |
Authors: | V Hansson E M Ritzén F S French S C Weddington S N Nayfeh E Reusch A Attramadal |
| |
Institution: | 1. University of North Carolina at Chapel Hill, U.S.A.;7. Karolinska Institutet, Stockholm, Sweden;71. Rikshospitalet, Oslo, Norway |
| |
Abstract: | Rabbit serum contains a specific androgen binding protein which can be separated from the corticosteroid binding globulin (CBG) in rabbit serum by sucrose gradient ultracentrifugation and polyacrylamide gel electrophoresis. It has a sedimentation constant of 4–5 S (mean 4.4 S) and high binding affinities for 5α-dihydrotestosterone, 5α-androstan-3α, 17β-diol and testosterone but negligible affinity for androstenedione, progesterone or corticosterone. Concentrations of the androgen binding protein expressed as 5α-dihydrotestosterone (DHT) binding capacity at saturation are higher in adult female (4.0 ± 0.3 μg DHT bound/100 ml) than in adult male sera (1.4 ± 0.8 μg DHT bound/100 ml). Immature male sera contain slightly higher amounts than adult females. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|