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Host genetic requirements for DNA release of lactococcal phage TP901-1 |
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| Authors: | Sofía Ruiz-Cruz Andrea Erazo Garzon Philip Kelleher Francesca Bottacini Solvej Østergaard Breum Horst Neve Knut J. Heller Finn K. Vogensen Simon Palussière Pascal Courtin Marie-Pierre Chapot-Chartier Evgeny Vinogradov Irina Sadovskaya Jennifer Mahony Douwe van Sinderen |
| Affiliation: | 1. School of Microbiology & APC Microbiome Ireland, University College Cork, Cork, Ireland;2. School of Microbiology & APC Microbiome Ireland, University College Cork, Cork, Ireland Department of Biological Sciences, Munster Technological University, Cork, Ireland;3. Section of Microbiology and Fermentation, Department of Food Science, Faculty of Science, University of Copenhagen, Frederiksberg, Denmark;4. Department of Microbiology and Biotechnology, Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Kiel, Germany Retired.;5. Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France;6. National Research Council Canada, Institute for Biological Sciences, Ottawa, Ontario, Canada;7. Equipe BPA, Université du Littoral-Côte d'Opale, Institut Charles Violette EA 7394 USC Anses, Boulogne-sur-mer, France |
| Abstract: | The first step in phage infection is the recognition of, and adsorption to, a receptor located on the host cell surface. This reversible host adsorption step is commonly followed by an irreversible event, which involves phage DNA delivery or release into the bacterial cytoplasm. The molecular components that trigger this latter event are unknown for most phages of Gram-positive bacteria. In the current study, we present a comparative genome analysis of three mutants of Lactococcus cremoris 3107, which are resistant to the P335 group phage TP901-1 due to mutations that affect TP901-1 DNA release. Through genetic complementation and phage infection assays, a predicted lactococcal three-component glycosylation system (TGS) was shown to be required for TP901-1 infection. Major cell wall saccharidic components were analysed, but no differences were found. However, heterologous gene expression experiments indicate that this TGS is involved in the glucosylation of a cell envelope-associated component that triggers TP901-1 DNA release. To date, a saccharide modification has not been implicated in the DNA delivery process of a Gram-positive infecting phage. |
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