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A polyhydroxyalkanoates bioprocess improvement case study based on four fed-batch feeding strategies |
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| Authors: | Maciej W. Guzik Gearóid F. Duane Shane T. Kenny Eoin Casey Paweł Mielcarek Magdalena Wojnarowska Kevin E. O’Connor |
| Affiliation: | 1. School of Biomolecular and Biomedical Sciences and BiOrbic Bioeconomy SFI Research Centre, UCD O’Brien Centre for Science, University College Dublin, Belfield, Dublin 4, Ireland;2. School of Chemical and Bioprocess Engineering, Engineering and Materials Science Centre, University College Dublin, Belfield, Dublin 4, Ireland;3. Department of Organizational Theory and Management, Poznań University of Economics and Business, al. Niepodległości 10, Poznań, 61-875 Poland;4. Department of Product Technology and Ecology, Cracow University of Economics, ul. Rakowicka 27, Kraków, 31-510 Poland |
| Abstract: | The modelling and optimization of a process for the production of the medium chain length polyhydroxyalkanoate (mcl-PHA) by the bacterium Pseudomonas putida KT2440 when fed a synthetic fatty acid mixture (SFAM) was investigated. Four novel feeding strategies were developed and tested using a constructed model and the optimum one implemented in further experiments. This strategy yielded a cell dry weight of 70.6 g l−1 in 25 h containing 38% PHA using SFAM at 5 l scale. A phosphate starvation strategy was implemented to improve PHA content, and this yielded 94.1 g l−1 in 25 h containing 56% PHA using SFAM at 5 l scale. The process was successfully operated at 20 l resulting in a cell dry weight of 91.2 g l−1 containing 65% PHA at the end of a 25-h incubation. |
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