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Chemical characterization of sialyl oligosaccharides in milk of the Japanese black bear, Ursus thibetanus japonicus
Authors:Urashima Tadasu  Nakamura Tadashi  Teramoto Kumi  Arai Ikichi  Saito Tadao  Komatsu Takeshi  Tsubota Toshio
Institution:aDepartment of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido, 080-8555, Japan;bDepartment of Bio Production, Graduate School of Agriculture, Tohoku University, Tsutsumidori-Amamiya machi 1-1, Aoba-Ku, Sendai, Miyagi 981-8555, Japan;cThe Institute of Japanese Black Bear in Ani, Mataginosato Bear Park, Uttoh, Ani-machi, Kita-Akita-Gun, Akita-Ken 018-4371, Japan;dDepartment of Veterinary Medicine, Faculty of Agriculture, Gifu University, Yanagido 1-1, Gifu 501-1193, Japan
Abstract:Sialyl oligosaccharides were separated from two samples of Japanese black bear milk by extraction with chloroform/methanol, gel filtration on Bio Gel P-2, ion exchange chromatography on DEAE-Sephadex A-50 and high-performance liquid chromatography (HPLC) on a TSK gel Amido-80 column. They were characterized by 1H-NMR spectroscopy. The structures of four sialyl oligosaccharides separated from the milk were the following:
Neu5Ac(α2-3)Gal(β1-4)Glc
Neu5Ac(α2-6)Gal(β1-4)GlcNAc(β1-3) Gal(α1-3)Gal(β1-4)Fuc(α1-3)]GlcNAc(β1-6) Gal(β1-4)Glc
Neu5Ac(α2-6)Gal(β1-4)GlcNAc(β1-3) Gal(α1-3)Fuc(α1-2)]Gal(β1-4)Fuc(α1-3)]GlcNAc(β1-6) Gal(β1-4)Glc
Neu5Ac(α2-6)Gal(β1-4)GlcNAc(β1-3)Neu5Ac(α2-6)Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc
Keywords: Japanese black bear milk; Milk oligosaccharides; Sialyl milk oligosaccharides; B antigen; α-Gal epitope; Ursidae; Ursus thibetanus japonicus; Japanese black bear
Keywords:Japanese black bear milk  Milk oligosaccharides  Sialyl milk oligosaccharides  B antigen  α  -Gal epitope  Ursidae  Ursus thibetanus japonicus  Japanese black bear
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