Possible involvement of protein kinase C in proliferation and differentiation of osteoblast-like cells |
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| Authors: | O Kozawa K Takatsuki K Kotake M Yoneda Y Oiso H Saito |
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| Institution: | First Department of Internal Medicine, Nagoya University School of Medicine, Japan. |
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| Abstract: | In cloned osteoblast-like cells, MC3T3-E1, 12-O-tetradecanoylphorbol-13-acetate (TPA), a protein kinase C activating phorbol ester, and 1-oleoyl-2-acetylglycerol (OAG), a specific activator for protein kinase C, stimulated DNA synthesis in a dose-dependent manner. Both TPA and OAG acted synergistically with insulin-like growth factor I to stimulate DNA synthesis. TPA as well as OAG suppressed the increase in alkaline phosphatase activity of MC3T3-E1 cells induced by parathyroid hormone. These results suggest that protein kinase C is involved in the process which directs osteoblast-like cells toward proliferation. |
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