维吾尔族正常黑胆质人群肠道菌群的分布情况分析

目的：了解维吾尔医学正常黑胆质人群肠道菌群分布情况、多样性并优势菌。方法：对健康人进行维吾尔医学体液分型并挑 取其中正常黑胆质人群，采集受检者粪便样品，提取总DNA，设计一对通用引物扩增16S rDNA 的V6～V8 可变区，扩增出来的 PCR产物稀释并进行变形梯度凝胶电泳DGGE，从DGGE 指纹图谱中选择条带，切胶回收、克隆、序列测定。结果：通过实验得到 了反映肠道菌群结构特征的DNA指纹图谱，从指纹图谱上选择一些特异性条带切下来回收，重新纯化扩增出来并测序，测出来 的基因序列在基因库进行比对检测相似性程度。最终用相似性程度大于95%以上的序列比对做出进化树了解菌群之间的亲缘 性。结论：正常黑胆质人群肠道菌群基因序列的亲缘性结果显示黑胆质人群肠道菌群具有丰富的多样性，其中肠道优势细菌乳酸 杆菌属GU269544.1 占优势。

Analysis of the Intestinal Bacterial Diversity in Normal SAVDA HILIT People of Uighur Nationality

Objective:To analyze the distribution diversity of intestinal flora and detect the dominant bacteria in normal Savda Hilits people of Uighur nationality.Methods:Healthy savda hilit were chosen by Uighur medicine and stool samples were collected from these groups. Then the total DNA was extracted, a pair of universal primers were designed to amplify the 16S rDNA V6 to V8 variable regions. Denaturing gradient gel electrophoresis (DGGE) of amplified sequences was performed, then the DGGE bands and sequencing was cloned.Results:The DNA fingerprinting reflecting the structural features of intestinal flora was obtained, some specific band sequencing were selected and cut from fingerprinting, the detected gene sequence was compared with the Genebank database on the Blast program, and make the evolutionary tree understanding of the relationship between intestinal microflora.Conclusion:Normal savda Hilit gut microbial gene sequence showed savda Hilit intestinal bacteria had rich diversity and the savda hilit gut microbial Lactobacillus (GU269544.1) had a height advantage.