Expression of the JIM8 cell wall epitope in carrot somatic embryogenesis |
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Authors: | Marcel A J Toonen Ed D L Schmidt Theo Hendriks Harrie A Verhoeven Ab van Kammen Sacco C de Vries |
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Institution: | (1) Department of Molecular Biology, Agricultural University Wageningen, Dreijenlaan 3, NL-6703 HA Wageningen, The Netherlands;(2) Centre of Plant Breeding and Reproduction Research (CPRO-DLO), Droevendaalsesteeg 1, P.O. Box 16, NL-6700 AA Wageningen, The Netherlands |
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Abstract: | Certain single cells in carrot (Daucus carota L.) suspension cultures react with the monoclonal antibody JIM8, and it has been proposed that these cells represent a transitional stage in somatic embryo formation. Shortly after isolation of the single cells by sieving, up to 80% of the cells react with JIM8. Within 4 d, JIM8 labelling becomes restricted to 1% of the single cells. To obtain evidence for the proposed correlation between expression of the JIM8 cell wall epitope and somatic embryo formation the developmental fate of carrot single cells labelled with JIM8 was determined by cell tracking. The results, obtained by recording 43 000 cells, show that only few JIM8-labelled cells give rise to embryos, and most somatic embryos develop from cells devoid of the JIM8 cell wall epitope. We therefore conclude that the presence of the JIM8 cell wall epitope does not coincide with the ability of single suspension cells to form embryos.Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- AGP
arabino galactan protein
- B5-0
Gamborg's B5 medium
- B5-0.2
Gamborg's B5 medium supplemented with 0.2 M 2,4-D
- FITC
fluoresceïn isothiocyanate
- PBS
phosphate-buffered saline |
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Keywords: | Arabinogalactan protein epitope Cell tracking Daucus Monoclonal antibody (JIM8) Somatic embryogenesis |
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