尿素氮-葡萄糖双功能分析仪的研究

由固定化脲酶、谷氨酸脱氢酶、谷氨酸氧化酶、葡萄糖氧化酶的复合酶膜组成的双电极系统,可以同时测定尿素氮和葡萄糖的含量,每次进样量为25μl,20s即可测定出尿素氮和葡萄糖的含量。在0～60mg/dl尿素氮、0～500mg/dl葡萄糖范围内具有良好的线性关系。连续测定20次的变异系数分别为1.02％和1.05％。酶膜使用寿命为两星期以上。此仪器可广泛应用于临床检验和体育训练中。

Study on Bifunctional Analyser of Urea-nitrogen and Glucose

The urea-nitrogen and glucose bifunctional analyser consisted of two electrodes, immobilized urease, glutamante oxidase, glutamate dehydrogenase and glucose oxidase can simultaneously determine the concentration of urea-nitrogen and glucose. The linear range is 0-60mg/dl for urea-nitrogen and 0 - 500mg/dl for glucose with a response of 20 seconds and 25ul sample injection. The coefficient of variation is 1. 02% and 1. 05% respectively in 20 assays. The enzyme membrances can be used continuously at 25C for two weeks. This bifunctional analyser can be used widely to determine urea-nitrogen and glucose at the same time in clinical and physical training.