陶瓷载体固定化酵母发酵动力学研究

固定化细胞和固定化酶一样,作为固液两相的非均相催化反应系统,其反应速度无疑受到内外扩散的影响。世界各国的化学工程学者在固定化酶反应动力学方面研究得较多,固定化细胞反应动力学研究虽有些报道,但主要集中在以海藻酸钙为载体的固定化系统,且载体形状为球形颗粒。对于其他材料的不同形状颗粒载体的固定化细胞动力学研究报道较少。陶瓷作为固定化细胞载体是我们研究的一种固定化细胞的新型载体，在机械强度，孔径大小，细胞与之结合牢固度及其稳定活性，再生性能方面有其特有的优越性^10]。为了给在生产实践中使用这种新型载体提供理论依据，本文采用球型陶瓷和拉西环陶瓷为载体固定化酵母，对它们的发酵动力学进行了比较研究。

Study on Fermentation Kinetics of the Yeast Cells Immobilized in the Carriers of Ceramics

Spherical and rashig ring ceramics carrier were used to immobilize yeast cells to study the kinetics of their fermentation. The results showed that the kinetics constants of fermentation of the cells immobilized in the ceramics carrier were influenced by the diffusion inside and outside the carrier. The kinetics constants measured are for spherical carrier. Michaelis constant (K,) = 5.3g/L, maximum velocity (Vm) =0. 49g - (g - h)-1, inhibition constant of substrate (Kiss) =278g/L, maximum level of product resistance (Pm) =120g/L; for Rashig ring carrier; K, = 4. 1g/L, Vn = 0. 55g - (g -h)-1,Kis=320g/L,Pm = 140g/L.