| 芍药花瓣总RNA的提取 |
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| 引用本文： | 黄凤兰,李长海,孙婷婷,高秀琴,胡宝忠.芍药花瓣总RNA的提取[J].生物技术通讯,2005,16(3):282-283. |
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| 作者姓名： | 黄凤兰 李长海 孙婷婷 高秀琴 胡宝忠 |
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| 作者单位： | 1. 东北农业大学,生命科学院,黑龙江,哈尔滨,150030
2. 黑龙江省森林植物园,黑龙江,哈尔滨,150030 |
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| 摘 要： | 用TRIzol法和改良的热硼酸盐法从芍药幼嫩花瓣中提取总RNA，总RNA的D260nm／D280nm值分别为1．803、1．974。琼脂糖电泳表明，用TRIzol法提取的RNA只有28S、18S2条带，带的亮度差，说明RNA有些降解；而用改良的热硼酸盐法提取的总RNA有28S、18S、5S3条带，带的亮度强，且28S是18S宽度的2倍左右，说明提取的RNA完整、未降解，可用于后续实验。改良的热硼酸盐法更适于从芍药花瓣中提取总RNA。
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| 关 键 词： | 总RNA 提取 花瓣 芍药 琼脂糖电泳 酸盐法 28S 18S 改良 亮度差 条带 降解 |
| 文章编号： | 1009-0002(2005)03-0282-02 |
| 修稿时间： | 2004年11月24 |
Eetraction of Total RNA in Petals of Paeonia lactiflora Pall |
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| HUANG Feng-lan,LI Chang-hai,SUN Ting-ting,Gao Xiu-qin,HU Bao-zhong.Eetraction of Total RNA in Petals of Paeonia lactiflora Pall[J].Letters in Biotechnology,2005,16(3):282-283. |
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| Authors: | HUANG Feng-lan LI Chang-hai SUN Ting-ting Gao Xiu-qin HU Bao-zhong |
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| Institution: | HUANG Feng-lan1,LI Chang-hai2,SUN Ting-ting1,GAO Xiu-qin2,HU Bao-zhong1 1. College of Life Science,Northeast Agricultural University,Harbin 150030, 2. Heilongjiang Forest Vivarium,Harbin 150030,China |
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| Abstract: | Total RNA from the tender petals of Paeonia lactiflora Pall was extracted by the methods of TRIzol and improved hot-borate. The results showed that the D260nm/D280nm of two methods were 1.803 and 1.974 respectively. After gelose electrophoresis, the RNA extracted by TRIzol only had two bands 28S and 18S, and the bands were dark, which meaned RNA is destroyed more or less; while the RNA extracted by improved hot-borate had three bands 28S, 18S and 5S, the bands were light, and the 28S was as two times length as the 18S, which meaned the extracted RNA was integrated enough to be used into the following experiment. The method of improved hot-borate is more suitable to extract total RNA from the tender petals of Paeonia lactiflora Pall. |
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| Keywords: | Paeonia lactiflora Pall TRIzol method improved hot-borate method total RNA extraction |
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