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Assessment of the Resistance to Uranium (VI) Exposure by Arthrobacter sp. Isolated from Hanford Site Soil
Authors:Yelena Katsenovich  Denny Carvajal  Rakesh Guduru  Leonel Lagos  Chen-Zhong Li
Affiliation:1. Applied Research Center , Florida International University , Miami , Florida , USA;2. Applied Research Center , Florida International University , Miami , Florida , USA;3. Nanobioengineering/Bioelectronic Lab, Department of Biomedical Engineering , Florida International University , Miami , Florida , USA;4. Nanobioengineering/Bioelectronic Lab, Department of Biomedical Engineering , Florida International University , Miami , Florida , USA
Abstract:
Production of nuclear fuel has resulted in hazardous waste streams that have contaminated the soil and groundwater. Arthrobacter strains, G975, G968, and G954 were used in the prescreening tests to evaluate their tolerance to UO2 2+ and investigate bacteria-U(VI) interactions under oxidizing pH-neutral conditions. Experiments have shown G975 is the fastest growing and the most uranium tolerant strain that removed about 90% of uranium from growth media. Atomic Force Microscopy images exhibited an irregular surface structure, which perhaps provided a larger surface area for uranium precipitation. The data indicate that aerobic heterotrophic bacteria may offer a solution to sequestering uranium in oxic conditions, which prevail in the vadose zone.
Keywords:Arthrobacter strains  viability  resistance  radionuclides  microscopy
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