Isolation and Characterization of Arsenic-Resistant Bacteria from Contaminated Water-Bodies in West Bengal,India |
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| Authors: | Ramansu Goswami Suprabhat Mukherjee Vipin Singh Rana Dhira Rani Saha Rajagopal Raman Pratap Kumar Padhy |
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| Institution: | 1. Immunobiology Laboratory, Department of Zoology, University of Delhi, Delhi, India;2. Department of Environmental Studies, Visva-Bharati University, Santiniketan, West Bengal, India;3. Parasitology Laboratory, Department of Zoology, Visva-Bharati University, Santiniketan, India;4. Gut Biology Laboratory, Department of Zoology, University of Delhi, Delhi, India;5. Microscopy Laboratory, National Institute of Cholera and Enteric Diseases, Beliaghata, Kolkata, India;6. Department of Environmental Studies, Visva-Bharati University, Santiniketan, West Bengal, India |
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| Abstract: | Bengal Basin is known for severe arsenic contamination. In the present study, we have isolated six bacteria from the arsenic contaminated surface water of Bengal Basin. 16S rDNA sequence analysis identified them as Microbacterium oleivorans, Acinetobacter soli, Acinetobacter venetianus, Acinetobacter junii, Acinetobacter baumannii, Acinetobacter calcoaceticus. All the isolates possess arsenic accumulation potential and high molecular weight plasmid (>10 kb). PCR amplification indicated the presence of arsenic-resistance genes (arsB and aoxB) either in the genome or plasmid or in both in the isolated bacteria (except in Acinetobacter venetianus). Exposure to arsenic affected bacterial growth and induced alteration in cytoplasmic membrane integrity. |
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| Keywords: | 16S rDNA arsB aoxB arsenic bacteria surface water |
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