In vitro propagation of cassava (Manihot esculenta Crantz) |
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| Authors: | Michael K. Smith Brenda J. Biggs Kenneth J. Scott |
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| Affiliation: | (1) Department of Biochemistry, University of Queensland, Q.4067 St. Lucia, Australia;(2) Present address: Redlands Horticultural Research Station, P.O. Box 327, Q. 4163 Cleveland, Australia |
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| Abstract: | A method is presented for the rapid in vitro propagation of cassava (Manihot esculenta Crantz). Nodal explants were induced to grow as multiple-shoot cultures on a medium containing 1.0  M 6-benzylamino purine (BAP), supplemented with 0.25 M  -naphthaleneacetic acid (NAA). Nodes were removed from the shoots after three weeks of growth and subcultured on fresh culture medium. An average of 7.0 nodes were produced from each explanted node after three weeks in culture. Nodal explants were transferred to a medium containing 2.5 M indole-3-butyric acid (IBA) to improve root initiation on the developing plantlets. Plant establishment was possible upon transfer to soil. In vitro propagation offers enhanced rates of multiplication over more conventional methods of propagation. In addition, in vitro propagation facilitates the storage and international exchange of cassava germplasm. |
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| Keywords: | Manihot esculenta cassava propagation in vitro propagation tissue culture |
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