Biogenesis of mitochondria. Phospholipid synthesis in vitro by yeast mitochondrial and microsomal fractions |
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| Authors: | Gary S Cobon Peter D Crowfoot and Anthony W Linnane |
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| Institution: | Department of Biochemistry, Monash University, Clayton, Vic. 3168, Australia |
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| Abstract: | The ability in vitro of yeast mitochondrial and microsomal fractions to synthesize lipid de novo was measured. The major phospholipids synthesized from sn-2-(3)H]glycerol 3-phosphate by the two microsomal fractions were phosphatidylserine, phosphatidylinositol and phosphatidic acid. The mitochondrial fraction, which had a higher specific activity for total glycerolipid synthesis, synthesized phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine and phosphatidic acid, together with smaller amounts of neutral lipids and diphosphatidylglycerol. Phosphatidylcholine synthesis from both S-adenosylMe-(14)C]methionine and CDP-Me-(14)C]choline appeared to be localized in the microsomal fraction. |
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