DNA binding factor GT-2 from Arabidopsis |
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Authors: | Robert M Kuhn Timothy Caspar Katayoon Dehesh Peter H Quail |
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Institution: | (1) Department of Plant Biology, University of California-Berkeley, 800 Buchanan St., 94710 Albany, CA, USA;(2) USDA Plant Gene Expression Center, 800 Buchanan St., 94710 Albany, CA, USA;(3) Present address: Sinsheimer Laboratories, University of California, 95064 Santa Cruz, CA, USA;(4) Present address: E. I. Du Pont de Nemours Experimental Station, 19880 Wilmington, DE, USA |
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Abstract: | Complementary DNA clones encoding a DNA-binding factor have been obtained from Arabidopsis by DNA hybridization with a GT-2 factor cDNA clone from rice. The GT-2 gene appears to be present as a single copy in the Arabidopsis genome and is transcribed as a 2.1 kb mRNA which is not light-regulated. The longest open reading frame in the sequenced clones predicts a protein of 65 kDa, beginning with the first in-frame methionine. The protein contains basic, acidic, and proline/glutamine-rich motifs and has significant amino acid sequence homology to the rice GT-2 factor, including three regions of 50–75 amino acids each of greater than 60% identity. Two of these regions are predicted to form similar trihelix structures postulated to be involved in selective binding to specific variations of a GT-box motif DNA sequence found in the promoter regions of several plant genes. Except for weak similarity to a tobacco GT-box binding factor, GT-1a/B2F, Arabidopsis GT-2 has no similarity to other sequences in the databases. DNA-binding studies show that Arabidopsis GT-2 has binding characteristics similar to those of the rice GT-2 factor, but dissimilar to those of the tobacco GT-1a/B2F factor. The data indicate that a DNA-binding factor containing domains of similar structure and target-sequence specificity has been conserved between monocots and dicots. |
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