Simultaneous measurements of DNA and protein content of microorganisms by flow cytometry |
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| Authors: | K.-J. Hutter H. E. Eipel |
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| Affiliation: | (1) Fraunhofer-Gesellschaft, Institut für Aerobiologie, D-5948 Schmallenberg, Federal Republic of Germany;(2) Biophysics Systems GmbH, Im Eichenböhl 24, D-6140 Bensheim 3, Federal Republic of Germany |
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| Abstract: | Summary The simultaneous measurement of protein and DNA content of yeast on a cell by cell basis is described. A problem associated with partially overlapping fluorescence emission bands of the two fluorochromes is discussed. The rapid flow cytometric assay will be useful to monitor cell growth in industrial fermentation processes.Abbreviations DNA Desoxyribonucleic acid - YEP yeast extract peptone - FITC fluorescein-iso-thiocyanate |
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