| RNA干扰对离体大鼠神经干细胞NgR基因表达的影响 |
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| 引用本文： | 王丰,朱悦. RNA干扰对离体大鼠神经干细胞NgR基因表达的影响[J]. 中国组织化学与细胞化学杂志, 2008, 17(1): 24-29. DOI: 10.3870/j.issn.1004-1850.2008.01.006 |
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| 作者姓名： | 王丰 朱悦 |
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| 作者单位： | 中国医科大学附属第一医院骨科,沈阳,110001 |
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| 摘 要： | 目的观察RNA干扰（RNA interference，RNAi）对离体大鼠神经干细胞的Nogo-66受体（Nogo-66 reeeptor，NgR）基因表达的影响。方法设计并合成3条大鼠NgR基因mRNA的小分子干扰RNA（small interfering RNA，siRNA）转染体外培养的神经干细胞，免疫荧光及Western blot检测NgR表达。结果siRNA可以成功的转染神经干细胞，3条siRNA不同程度的阻断了神经干细胞的NgR基因表达，阻断效率随时间延长逐渐下降。阻断效果最好的1条siRNA在转染后第5d仍能保持（85．22±3．1）％高阻断效率。结论应用RNAi可以高效率的使大鼠神经干细胞的NgR基因表达沉默。
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| 关 键 词： | RNA干扰 神经干细胞 Nogo-66受体 |
| 修稿时间： | 2007-07-02 |
EFFECT OF RNAi ON THE NgR GENE EXPRESSION OF RAT NEURAL STEM CELLS IN VITRO |
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| Wang Feng,Zhu Yue. EFFECT OF RNAi ON THE NgR GENE EXPRESSION OF RAT NEURAL STEM CELLS IN VITRO[J]. Chinese Journal of Histochemistry and Cytochemistry, 2008, 17(1): 24-29. DOI: 10.3870/j.issn.1004-1850.2008.01.006 |
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| Authors: | Wang Feng Zhu Yue |
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| Affiliation: | Wang Feng,Zhu Yue*(Department of Orthopedics,The First Affiliated Hospital,China Medical University,Shenyang 110001,China) |
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| Abstract: | Objective To observe the effect of RNAi on the NgR gene expression of neural stem cells derived from the rat spinal cord in vitro.Methods Three siRNAs were disigned and synthesized according to the mRNA sequence of rat NgR gene,and transfect to neural stem cells in culture.The NgR gene expression was detectecl by immunofluoresence and Western blot.Results The NgR gene was knocked down by the three siRNAs in different degrees.The knock-down efficiency decreased with time after transfection.The most effective... |
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| Keywords: | RNA interference Neural stem cell Nogo-66 receptor |
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