Sugarcane molasses and yeast powder used in the Fructooligosaccharides production by Aspergillus japonicus-FCL 119T and Aspergillus niger ATCC 20611 |
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Authors: | Claudia Dorta Rubens Cruz Pedro de Oliva-Neto Danilo José Camargo Moura |
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Institution: | 1. Departamento de Ciências Biológicas-F.C.L., Universidade Estadual Paulista-UNESP, Assis, SP, Av. Dom Ant?nio, 2100, S?o Paulo, CEP 19800000, Brazil
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Abstract: | Different concentrations of sucrose (3–25% w/v) and peptone (2–5% w/v) were studied in the formulation of media during the cultivation of Aspergillus japonicus-FCL 119T and Aspergillus niger ATCC 20611. Moreover, cane molasses (3.5–17.5% w/v total sugar) and yeast powder (1.5–5% w/v) were used as alternative nutrients for both strains’ cultivation. These media were formulated for analysis of cellular growth, β-Fructosyltransferase and Fructooligosaccharides (FOS) production. Transfructosylating activity (U
t
) and FOS production were analyzed by HPLC. The highest enzyme production by both the strains was 3% (w/v) sucrose and 3% (w/v) peptone, or 3.5% (w/v) total sugars present in cane molasses and 1.5% (w/v) yeast powder. Cane molasses and yeast powder were as good as sucrose and peptone in the enzyme and FOS (around 60% w/w) production by studied strains. |
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Keywords: | Fructooligosaccharides Aspergillus japonicus Aspergillus niger Cane molasses Yeast powder |
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