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Potato tuber proteomics: comparison of two complementary extraction methods designed for 2-DE of acidic proteins |
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| Authors: | Delaplace Pierre van der Wal Froukje Dierick Jean-François Cordewener Jan H G Fauconnier Marie-Laure du Jardin Patrick America Antoine H P |
| Institution: | Plant Biology Unit, Gembloux Agricultural University, Gembloux, Belgium. delaplace.p@fsagx.ac.be |
| Abstract: | Two protein extraction procedures were tested in order to remove interfering compounds prior to 2-DE of potato tubers. These methods using SDS lysis buffer and phenol-phase extraction were compared regarding the quality of the resulting 2-D gel. While the resolution of SDS extracts on semipreparative gels seems better, both methods lead to similar extraction yields and total number of spots. The procedures are complementary regarding the Mr range of preferentially extracted proteins. |
| Keywords: | Extraction method Phenol Plant proteomics SDS Two‐dimensional gel electrophoresis |
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