Measurement of DNA content and cell volume in senescent human fibroblasts utilizing flow multiparameter single cell analysis |
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| Authors: | E. L. Schneider B. J. Fowlkes |
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| Affiliation: | 1. Genetics Laboratory, Department of Biochemistry, University of Oxford. Oxford OX1 3QU, UK;2. School of Medicine, Washington University, St Louis, MO 63110, USA |
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| Abstract: | Cell cycle analysis of senescent cultured human fibroblasts by flow cytofluorometry reveals an increased proportion of cells in the G 1 period. An increased variation as well as a slight decrease in cellular DNA contents were observed in both G 1 and G 2 + M senescent cells. Utilizing gated single parameter analysis, the increased cell volumes observed in these senescent fibroblasts were demonstrated to be present in G 1 as well as G2 + M cells. |
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