The growth form of the inducing microorganism and chitin addition affect mycolytic enzyme production byTrichoderma harzianum |
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| Authors: | P Žnidaršič A Pavko R Komel |
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| Institution: | (1) Department of Chemistry and Chemical Technology, University of Ljubljana, A ker eva 5, 61000 Ljubljana, Slovenia;(2) Institute of Biochemistry, Medical Faculty, Ljubljana, Slovenia |
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| Abstract: | The effect of the growth form of the inducing microorganism on specificTrichoderma harzianum mycolytic enzyme production was studied. The pelleted form ofRhizopus nigricans gave a better product concerning protoplast formation ability. The maximum yield of protoplasts from the target fungusCochliobolus lunatus was 1×108 ml–1. Analysis of individual specific enzyme activities inTrichoderma mycolytic enzyme preparations confirms the importance of high chitinase and low protease activity for high protoplast yields. Supplementation of the production medium with chitin increased the chitinase activity in theTrichoderma exoenzyme mixture. |
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| Keywords: | mycolytic enzymes Trichoderma harzianum protoplasts Rhizopus nigricans induction |
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