去卵巢致骨质疏松症大鼠腺垂体FS细胞中Cx43、S-100蛋白表达--激光扫描共聚焦显微镜观察

检测间隙连接蛋白Cx43、神经组织蛋白S-100在去卵巢致骨质疏松症(OVX-OP)大鼠腺垂体滤泡星形细胞(FS细胞)中的表达.实验采用10月龄未孕产SD雌性大鼠40只,随机均分为卵巢切除组(OVX组,n=20)和假性手术对照组(Sham组,n=20).于术后6周末用双能X线骨吸收测量法(DEXA)测量大鼠全身及腰椎4-6(L4-6)骨密度(BMD).取两组大鼠垂体,制成连续切片.应用FITC标记的IgG探针,对腺垂体组织中Cx43和S-100进行间接免疫荧光染色,并用激光扫描共聚焦显微镜(LSCM)定位和定量分析腺垂体FS细胞中Cx43、S-100的表达.结果发现,术后6周末OVX组大鼠全身及L4-6BMD均明显低于Sham组值(P<0.01,P<0.01).Cx43阳性荧光反应主要定位于相邻的FS细胞的胞浆中和/或胞膜上.OVX组Cx43阳性表达荧光强度和表达阳性率均显著低于Sham组(P<0.01).S-100蛋白表达定位于FS细胞的胞浆中,两组间S-100阳性表达荧光强度和表达阳性率无显著差异(P>0.05).本研究提示,SD大鼠OVX术后6周出现骨质疏松变化;OVX大鼠腺垂体FS细胞数量无明显变化、而Cx43表达显著下降,后者的变化可能与大鼠OVX-OP发生相关.

EXPRESSION OF CONNEXIN 43 AND NERVE TISSUE PROTEIN S-100 IN ADENOHYPOPHY SEALFOLLICULO-STELLATE CELLS IN RAT MODEL OF OVARIECTOMIZED OSTEOPOROSIS --A STUDY WITH LASER SCANNING CONFOCAL MICROSCOPY

To investigate the expression of gap junction protein connexin 43 (Cx43) and nerve tissue protein S 100(S 100) in adenohypophyseal folliculo stellate cells (FSCs) in the ovariectomized osteoporosis (OVX OP), osteoporosis models of rats were obtained by bilateral ovariectomy. 40 female rats were equally devided into an ovariectomized group (OVX group) and asham operation control group (sham group). Total skeletal bone mineral density (BMD) and spinal(L 4-6 )BMD of each rat were measured with dual energy X ray absorptiometry (DEXA) at the end of 6 weeks after operation. The expression of Cx43 and S 100 in adenohypophysis was detected by immunofluorescent histochemistry and laser scanning confocal microscopy (LSCM). The results showed that the total skeletal BMD and L 4-6 BMD of rats in the OVX group were significantly lower than those in the sham group ( P <0 01, P <0 01). The fluorescence of Cx43 distributed in the cytoplasm and along the cell membrane of FSCs. Both Cx43 fluorescence intensity and Cx43 positive cell ratio were lower in the OVX group than the in Sham group ( P <0 01), while the fluorescence and positive rate of S 100 of FSCs in the OVX group had no discrepancy compared with sham group. These results indicated that the reduction of Cx43 expression in FSCs after ovariectomy could presumably play a role in ovariectomy induced osteoporosis.