Experimental determination of the molar differential extinction coefficient of P700 |
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| Affiliation: | 1. School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China;2. State Key Laboratory of Chemical Engineering, Tianjin University, Tianjin 300350, China;3. Chemical Engineering Research Center, Tianjin University, Tianjin 300350, China;1. Biotopic, Nyvej 33B, 5762 Vester Skerninge, Denmark;2. Proviron, G. Giliotstraat 60, 2620 Hemiksem, Belgium;3. Operations Research and Logistics, Wageningen University & Research, PO Box 8130, 6700 EW, Wageningen, the Netherlands;4. Greenhouse Horticulture, Wageningen University & Research, PO Box 16, 6700 AA, Wageningen, the Netherlands;5. Bioprocess Engineering, Algaeparc, Wageningen University & Research, PO Box 16, 6700 AA, Wageningen, the Netherlands;6. Faculty of Biosciences and Aquaculture, Nord University, N-8049 Bodø, Norway;1. Beckman Institute, University of Illinois at Urbana-Champaign, 405 N. Mathews Ave, Urbana, IL 61801, USA;2. Department of Physics, University of Illinois at Urbana-Champaign, 1110 W Green St, Urbana, IL 61801, USA;3. School of Applied and Engineering Physics, Cornell University, 271 Clark Hall, Ithaca, New York 14853, USA;4. Kavli Institute at Cornell for Nanoscale Sciences, 420 Physical Sciences Building, Ithaca, New York 14853, USA;5. Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield, South Yorkshire S10 2TN, UK |
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| Abstract: | The molar differential extinction coefficients of P700 at 435 and 703 nm have been determined by using a directly coupled reaction in the dark involving the reduction of photooxidized P700 and the oxidation of reduced cytochromes.From the coupled oxidation of mammalian cytochrome c by Anabaena high-P700 particles, the molar differential extinction coefficients of P700 at 435 and 703 nm are calculated to be 8.6·104 and 1.20·105 M−1·cm−1, respectively, and the ratio of the red to blue band heights is 1.4.From the coupled oxidation of Euglena cytochrome-552 by Triton-fractionated pigment system 1 subchloroplast particles enriched in P700, the calculated molar differential extinction coefficients of P700 at 435 and 703 nm are very close to the values given above.Using an absorbance decrease at 580 nm as a measure of the photoreduction of dichlorophenolindophenol by Triton-fractionated pigment system 1 subchloroplast particles enriched in P700 tends to yield a low extinction value because of other absorbance changes which occur at this wavelength and the non-reproducibility of the values obtained.Comparisons are made between the extinction values of P700 and the corresponding extinction values of the bacteriochlorophyll reaction centers in photosynthetic bacteria. |
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