Blue photobiomodulation LED therapy impacts SARS-CoV-2 by limiting its replication in Vero cells |
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| Authors: | Luisa Zupin Rossella Gratton Francesco Fontana Libera Clemente Lorella Pascolo Maurizio Ruscio Sergio Crovella |
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| Affiliation: | 1. Medical Genetics, Institute for Maternal and Child Health—IRCCS “Burlo Garofolo”, Trieste, Italy;2. Division of Laboratory Medicine, University Hospital Giuliano Isontina (ASU GI), Trieste, Italy;3. Obstetrics and Gynecology, Institute for Maternal and Child Health—IRCCS “Burlo Garofolo”, Trieste, Italy;4. Department of Biological and Environmental Sciences, College of Arts and Sciences, University of Qatar, Doha, Qatar |
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| Abstract: | The study of any intervention able to counteract SARS-CoV-2 pandemic is considerably envisaged. It was previously shown, in in vitro models of infections, that the LED blue light is able to decrease the viral load of HSV-1 and ZIKV. In our study, LED photobiomodulation therapy (PBMT) at blue wavelengths (450, 454 and 470 nm) was tested in an in vitro model of SARS-CoV-2 infection, employing three experimental settings: SARS-CoV-2 was irradiated and then transferred to cells; already infected cells were irradiated; cells were irradiated prior to infection. A decrement of the viral load was observed when previously infected cells were irradiated with all three tested wavelengths and relevant effects were registered especially at 48 hours post-infection, possibly suggesting that the blue light could interfere with the intracellular viral replication machinery. Our in vitro findings could represent the starting point for translational applications of PBMT as a supportive approach to fight SARS-CoV-2. |
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| Keywords: | blue LED photobiomodulation therapy SARS-CoV-2 Vero E6 cell line |
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