Picric acid-formaldehyde fixation for immunoferritin studies |
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| Authors: | Lidia Accinni Konrad C Hsu Hermina Spiele Cesare De Martino |
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| Institution: | (1) Clinica Medica 2, University of Rome, Rome, Italy;(2) Department of Microbiology, Columbia University, New York, USA;(3) Department of Electron Microscopy, The Netherlands Cancer Institute, Amsterdam, The Netherlands;(4) Institute Regina Elena for Cancer Research, Rome, Italy |
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| Abstract: | Summary Picric acid-formaldehyde (PAF) fixative has been compared to other fixatives commonly employed in electron microscopy in order to check its ability to be used in immunoferritin studies. From the results obtained PAF seems to offer very good preservation of the tissue fine structure together with satisfactory detection of immune reactions at ultrastructural level.This investigation was supported by Grants CT 72.00900.04, 73.00449.04, 73.00550.04, from the Consiglio Nazionale delle Ricerche, Italy; and by United States Public Health Service Grants AM-13200 and AM-14928. |
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