Purification and crystallization of rat liver fatty acid synthetase |
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| Authors: | Tracy C. Linn |
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| Affiliation: | 1. Pre-Clinical Science Unit, Veterans Administration Medical Center, Dallas, Texas 75216, U.S.A.;2. The Department of Biochemistry, University of Texas Health Science Center, Dallas, Texas 75235 U.S.A. |
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| Abstract: | A purification procedure for rat liver fatty acid synthetase has been developed using polyethylene glycol. This procedure results in high yields of the enzyme which is essentially free of endogenous proteolytic nicking and also free of any contaminating proteases. The fatty acid synthetase obtained has a specific activity range of 1.8–2.1 measured at 25 °C and is stable at 4 °C for a few weeks and indefinitely when frozen. Approximately 1 mg of enzyme can be obtained per gram of induced rat liver. The enzyme is pure as determined by sodium dodecyl sulfate-gel electrophoresis, sedimentation velocity, and immunoelectrophoresis. The first crystallization of rat liver fatty acid synthetase is also reported. |
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| Keywords: | To whom correspondence should be addressed. |
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