Full-length infectious clone of a low passage dengue virus serotype 2
from Brazil |
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| Authors: | Jefferson José da Silva Santos Tereza Magalh?es José Valter Joaquim Silva Junior Andréa Nazaré Monteiro Rangel da Silva Marli Tenório Cordeiro Laura Helena Vega Gonzales Gil |
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| Affiliation: | 1.Fundação Oswaldo Cruz, Centro de Pesquisas Aggeu Magalhães, Laboratório de Virologia e Terapia Experimental, Recife, PE, Brasil;2.Departamento de Saúde do Estado de Pernambuco, Laboratório Central de Saúde Pública, Recife, PE, Brasil |
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| Abstract: | ![]()
Full-length dengue virus (DENV) cDNA clones are an invaluable tool for many studies,including those on the development of attenuated or chimeric vaccines and onhost-virus interactions. Furthermore, the importance of low passage DENV infectiousclones should be highlighted, as these may harbour critical and uniquestrain-specific viral components from field-circulating isolates. The successfulconstruction of a functional Brazilian low passage DENV serotype 2 full-length clonethrough homologous recombination reported here supports the use of a strategy thathas been shown to be highly useful by our group for the development of flavivirusinfectious clones and replicons. |
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| Keywords: | infectious clone yeast homologous recombination flavivirus |
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