Use of Centrifugal Filter Devices to Concentrate Dengue Virus in Mosquito per os Infection Experiments |
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Authors: | Vaea Richard Jér?me Viallon Van-Mai Cao-Lormeau |
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Institution: | Institut Louis Malardé, Papeete, French Polynesia.; Centro de Pesquisas René Rachou, BRAZIL, |
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Abstract: | Dengue virus (DENV) is an arbovirus transmitted to humans by the bite of infected Aedes mosquitoes. Experimental per os infection of mosquitoes with DENV is usually a preliminary step in virus/vector studies but it requires being able to prepare artificial blood-meals with high virus titers. We report here the convenient use of centrifugal filter devices to quickly concentrate DENV particles in cell-culture supernatants. The median viral titer in concentrated-supernatants was 8.50 log10 TCID50/mL. By using these DENV concentrated-supernatants to prepare infectious blood-meals in Aedes aegypti per os infection experiments, we obtained a mean mosquito-infection rate of 94%. We also evaluated the use of centrifugal filter devices to recover DENV particles from non-infectious blood-meals presented to infected mosquitoes through a feeding membrane to collect their saliva. |
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