Embryonic Hematopoietic Progenitor Cells Reside in Muscle before Bone Marrow Hematopoiesis |
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| Authors: | Yuka Tanaka Tomoko Inoue-Yokoo Kasem Kulkeaw Chiyo Yanagi-Mizuochi Senji Shirasawa Yoichi Nakanishi Daisuke Sugiyama |
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| Institution: | 1. Center for Advanced Medical Innovation, Kyushu University, Fukuoka, Japan.; 2. Department of Cell Biology, Faculty of Medicine, Fukuoka University, Fukuoka Japan.; 3. Department of Research and Development of Next Generation Medicine, Kyushu University Faculty of Medical Sciences, Fukuoka, Japan.; 4. Center for Clinical and Translational Research, Kyushu University Hospital, Fukuoka, Japan.; National Institutes of Health, UNITED STATES, |
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| Abstract: | In mice, hematopoietic cells home to bone marrow from fetal liver prenatally. To elucidate mechanisms underlying homing, we performed immunohistochemistry with the hematopoietic cell marker c-Kit, and observed c-Kit(+) cells localized inside muscle surrounding bone after 14.5 days post coitum. Flow cytometric analysis showed that CD45(+) c-Kit(+) hematopoietic cells were more abundant in muscle than in bone marrow between 14.5 and 17.5 days post coitum, peaking at 16.5 days post coitum. CD45(+) c-Kit(+) cells in muscle at 16.5 days post coitum exhibited higher expression of Gata2, among several hematopoietic genes, than did fetal liver or bone marrow cells. Colony formation assays revealed that muscle hematopoietic cells possess hematopoietic progenitor activity. Furthermore, exo utero transplantation revealed that fetal liver hematopoietic progenitor cells home to muscle and then to BM. Our findings demonstrate that hematopoietic progenitor cell homing occurs earlier than previously reported and that hematopoietic progenitor cells reside in muscle tissue before bone marrow hematopoiesis occurs during mouse embryogenesis. |
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