Dynamic light scattering: a practical guide and applications in biomedical sciences |
| |
Authors: | Jörg Stetefeld Sean A. McKenna Trushar R. Patel |
| |
Affiliation: | 1.Department of Chemistry,University of Manitoba,Winnipeg,Canada;2.Department of Biochemistry and Medical Genetics,University of Manitoba,Winnipeg,Canada;3.School of Biosciences,University of Birmingham,Birmingham,UK;4.Alberta RNA Research and Training Institute, Department of Chemistry & Biochemistry,University of Lethbridge,Lethbridge,Canada |
| |
Abstract: | Dynamic light scattering (DLS), also known as photon correlation spectroscopy (PCS), is a very powerful tool for studying the diffusion behaviour of macromolecules in solution. The diffusion coefficient, and hence the hydrodynamic radii calculated from it, depends on the size and shape of macromolecules. In this review, we provide evidence of the usefulness of DLS to study the homogeneity of proteins, nucleic acids, and complexes of protein–protein or protein–nucleic acid preparations, as well as to study protein–small molecule interactions. Further, we provide examples of DLS’s application both as a complementary method to analytical ultracentrifugation studies and as a screening tool to validate solution scattering models using determined hydrodynamic radii. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|