| 香椿子正丁醇提取物通过激活Nrf2改善高糖引起的肾小球内皮细胞氧化应激 |
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| 引用本文： | 仲玉鑫,胡珊,王婷婷,张雨淼,李万忠,于丽,刘雨清,张红霞.香椿子正丁醇提取物通过激活Nrf2改善高糖引起的肾小球内皮细胞氧化应激[J].中国组织化学与细胞化学杂志,2019(3):197-203. |
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| 作者姓名： | 仲玉鑫 胡珊 王婷婷 张雨淼 李万忠 于丽 刘雨清 张红霞 |
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| 作者单位： | 潍坊医学院临床医学院临床病理学系;潍坊医学院神经疾病与再生修复实验室;潍坊医学院组织学与胚胎学教研室;潍坊医学院附属医院肾内科;潍坊医学院药学院药剂学教研室 |
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| 基金项目： | 山东省自然科学基金(ZR2018MH040);山东省自然科学基金(ZR2018MC012);潍坊医学院博士启动基金项目(2017BSQD23);山东省高等学校优秀骨干教师国际合作培养项目经费资助 |
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| 摘 要： | 目的探讨香椿子正丁醇提取物(n-butyl alcohol extract of toona sinensis,NBAE)对高糖引起的肾小球内皮细胞氧化应激的影响及机制。方法分别以高糖(high glucose,HG)、HG+NBAE刺激人肾小球内皮细胞,采用DCFDA法检测细胞内活性氧(reactive oxygenspecies,ROS)生成情况,Nitrate/Nitrite Colorimetric法检测细胞内NO的含量,Western blot检测Nrf2、NQO1及HO-1的蛋白表达,免疫荧光方法检测Nrf2、P47phox在细胞内的定位及表达。结果高糖刺激肾小球内皮细胞后出现氧化应激损伤,ROS升高,NO减少,p47phox表达量增高,Nrf2及下游蛋白NQO1、HO-1表达减少;NBAE可明显提高Nrf2的表达,并增加下游蛋白NQO1和HO-1的表达,从而抑制高糖导致的ROS升高,抑制p47phox的表达,并稳定NO的含量。结论NBAE通过激活Nrf2及其下游靶蛋白来改善高糖对肾小球内皮细胞造成的氧化应激损伤。
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| 关 键 词： | 香椿子正丁醇提取物 NRF2 肾小球内皮细胞 氧化应激 |
N-butyl alcohol extract of toona sinensis attenuates the oxidative stress of glomerular endothelial cells induced by high glucose through activating Nrf2 |
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| Zhong Yuxin,Hu Shan,Wang Tingting,Zhang Yumiao,Li Wanzhong,Yu Li,Liu Yuqing,Zhang Hongxia.N-butyl alcohol extract of toona sinensis attenuates the oxidative stress of glomerular endothelial cells induced by high glucose through activating Nrf2[J].Chinese Journal of Histochemistry and Cytochemistry,2019(3):197-203. |
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| Authors: | Zhong Yuxin Hu Shan Wang Tingting Zhang Yumiao Li Wanzhong Yu Li Liu Yuqing Zhang Hongxia |
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| Institution: | (Department of Clinic Pathology,School of Clinical Medicine,Weifang Medical University,Weifang 261053,China;Lab of Neurologic disorders and regenerative,Weifang Medical University,Weifang 261053,China;Department of Histology and Embryology,School of Clinical Medicine,Weifang Medical University,Weifang 261053,China;Department of Nephrology,Affiliated Hospital of Weifang Medical University,Weifang Medical University,Weifang 261053,China;Department of Pharmaceutics,School of Pharmacy,Weifang Medical University,Weifang 261053,China) |
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| Abstract: | Zhong Yuxin;Hu Shan;Wang Tingting;Zhang Yumiao;Li Wanzhong;Yu Li;Liu Yuqing;Zhang Hongxia(Department of Clinic Pathology,School of Clinical Medicine,Weifang Medical University,Weifang 261053,China;Lab of Neurologic disorders and regenerative,Weifang Medical University,Weifang 261053,China;Department of Histology and Embryology,School of Clinical Medicine,Weifang Medical University,Weifang 261053,China;Department of Nephrology,Affiliated Hospital of Weifang Medical University,Weifang Medical University,Weifang 261053,China;Department of Pharmaceutics,School of Pharmacy,Weifang Medical University,Weifang 261053,China) |
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| Keywords: | N-butyl alcohol extract of Toona Sinensis NF-E2-related factor 2(Nrf2) glomerular endothelial cells oxidative stress |
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