Permethylation and tandem mass spectrometry of oligosaccharides having free hexosamine: analysis of the glycoinositol phospholipid anchor glycan from the scrapie prion protein |
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| Authors: | M A Baldwin N Stahl L G Reinders B W Gibson S B Prusiner A L Burlingame |
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| Affiliation: | Department of Pharmaceutical Chemistry, University of California, San Francisco 94143. |
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| Abstract: | Permethylation of the glycan isolated from the glycoinositol phospholipid (GPI) anchor of the scrapie prion protein (PrPSc) trimethylates a free hexosamine to form a quarternary ammonium salt, substantially increasing the sensitivity for analysis by mass spectrometry. This derivatization induces specific fragmentation reactions in collision-induced dissociation spectra obtained on a four-sector tandem mass spectrometer, identifying the branching pattern of the PrPSc GPI glycan. |
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