Effect of vaccinations with recombinant fusion proteins on Ancylostoma caninum habitat selection in the canine intestine |
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Authors: | Hotez Peter J Ashcom James Bin Zhan Bethony Jeffrey Williamson Angela Hawdon John M Jianjun Feng Dobardzic Azra Rizo Ivania Bolden Janelle Jin Qun Yan Wang Dobardzic Reshad Chung-Debose Sophia Crowell Melissa Datu Bennett Delaney Angela Dragonovski Dilyan Jiang Yang Yueyuan Liu Ghosh Kashinath Loukas Alex Brandt Walter Russell Philip K Zook Bernard C |
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Institution: | Department of Microbiology and Tropical Medicine, Sabin Vaccine Institute, George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037, USA. |
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Abstract: | Laboratory dogs were vaccinated subcutaneously with 3 different recombinant fusion proteins, each precipitated with alum or calcium phosphate. The vaccinated dogs were then challenged orally with 400 third-stage infective larvae (L3) of the canine hookworm, Ancylostoma caninum. The 3 A. caninum antigens selected were Ac-TMP, an adult-specific secreted tissue inhibitor of metalloproteases; Ac-AP, an adult-specific secreted factor Xa serine protease inhibitor anticoagulant; and Ac-ARR-1, a cathepsin D-like aspartic protease. Each of the 3 groups comprised 6 male beagles (8 +/- 1 wk of age). A fourth group comprised control dogs injected with alum. All of the dogs vaccinated with Ac-TMP or Ac-APR-1 exhibited a vigorous antigen-specific antibody response, whereas only a single dog vaccinated with Ac-AP developed an antibody response. Dogs with circulating antibody responses exhibited 4.5-18% reduction in the numbers of adult hookworms recovered from the small intestines at necropsy, relative to alum-injected dogs. In contrast, there was a concomitant increase in the number of adult hookworms recovered from the colon. The increase in colonic hookworms was as high as 500%, relative to alum-injected dogs. Female adult hookworms were more likely to migrate into the colon than were males. Anti-enzyme and anti-enzyme inhibitor antibodies correlated with an alteration in adult hookworm habitat selection in the canine gastroinntestinal tract. |
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