p40phox as an alternative organizer to p47phox in Nox2 activation: a new mechanism involving an interaction with p22phox |
| |
| Authors: | Tamura Minoru Shiozaki Iichiro Ono Shohei Miyano Kei Kunihiro Sachio Sasaki Takayuki |
| |
| Affiliation: | Department of Applied Chemistry, Graduate School of Science and Engineering, Ehime University, Matsuyama, Ehime 790-8577, Japan. tamura@eng.ehime-u.ac.jp |
| |
| Abstract: | p40(phox) activated phagocyte NADPH oxidase without p47(phox) in a cell-free system consisting of p67(phox), Rac and cytochrome b(558) relipidated with phosphatidylinositol 3-phosphate. The activation reached to 70% of that by p47(phox). Addition of p47(phox) slightly increased the activation, but not additively. p40(phox) improved the efficiency of p67(phox) in the activation. The C-terminus-truncated p67(phox), p40(phox)(D289A), p40(phox)(R58A), or p40(phox)(W207R) showed an impaired activation. A peptide corresponding to the p22(phox) Pro-rich region suppressed the activation, and far-western blotting revealed its interaction with p40(phox) SH3 domain. Thus, p40(phox) can substitute for p47(phox) in the activation, interacting with p22(phox) and p67(phox) through their specific regions. |
| |
| Keywords: | cyt.b558, flavocytochrome b558 GST, glutathione-S-transferase PI 3-P, phosphatidylinositol 3-phosphate PI, phosphatidylinositol p40SH3, p40phox SH3 domain p47SH3, p47phox SH3 domain p22PR, p22phox Pro-rich region AIR, autoinhibitory region |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
