Green fluorescent protein as a reporter system in the transformation of barley cultivars |
| |
| Authors: | Staffan Ahlandsberg P Sathish Chuanxin Sun Christer Jansson |
| |
| Institution: | Department of Plant Biology, The Genetic Center, The Swedish University of Agricultural Sciences, PO Box 7080, S-75007 Ukppsala, Sweden |
| |
| Abstract: | A cereal transformation vector, pN1473, containing the strong constitutive rice actin promoter Act-1 , a multiple cloning site, and the nos terminator, was constructed. Fusion of a plant-optimized gfp gene to Act-1 in pN1473 resulted in the vector pN1473GFP. To assess the suitability of pN1473, and GFP as a reporter system in barley transformation, two barley cultivars (Baronesse and Golden Promise) were transformed by microprojectile bombardment. Transient gfp expression in transformed embryogenic callus material was detectable by fluorescence microscopy less than 12 h after transformation. The presence of the gfp gene in callus and regenerated plantlets was confirmed by PCR amplification and DNA gel-blot analysis. |
| |
| Keywords: | |
|
|
