| 有机溶剂对HPLC测定头孢菌素C含量的影响 |
| |
| 引用本文： | 赵明珠,赵凤生. 有机溶剂对HPLC测定头孢菌素C含量的影响[J]. 工业微生物, 2010, 40(3): 25-28. DOI: 10.3969/j.issn.1001-6678.2010.03.005 |
| |
| 作者姓名： | 赵明珠 赵凤生 |
| |
| 作者单位： | 上海交通大学药学院,上海,200240;上海交通大学药学院,上海,200240 |
| |
| 摘 要： | 考察了10种水溶性有机溶剂对HPLC测定头孢菌素C含量的影响,采用C_(18)色谱柱,流动相为磷酸二氢钠-甲醇(90:10),波长254 nm。发现不同的有机溶剂有不同程度的影响,其中乙醇的影响最大;乙醇浓度越高,峰面积就越小。将含有乙醇的头孢菌素C溶液稀释到一定程度,能够有效消除乙醇的影响。稀释法具有较好的精密度、重复性和回收率,为在工业生产中准确测定头孢菌素C的含量提供了依据。
|
| 关 键 词： | 头孢菌素C 高效液相色谱 有机溶剂 乙醇 |
Influence of organic solvents on determination of cephalosporin C by HPLC |
| |
| ZHAO Ming-zhu,ZHAO Feng-sheng. Influence of organic solvents on determination of cephalosporin C by HPLC[J]. Industrial Microbiology, 2010, 40(3): 25-28. DOI: 10.3969/j.issn.1001-6678.2010.03.005 |
| |
| Authors: | ZHAO Ming-zhu ZHAO Feng-sheng |
| |
| Affiliation: | (School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, P. R. China) |
| |
| Abstract: | The influences of ten kinds of organic solvents on the determination of cephalosporin C by HPLC were investigated. Using C18 reverse phase column, mobile phase was phosphate buffer-methanol （90：10） and the detection wavelength was 254 nm. The results showed that different organic solvents influenced the determination of eephalosporin C by HPLC to different extents, among them ethanol had the greatest impact. The higher of the ethanol concentration, the less peak area of cephalosporin C was. It could effectively eliminate the influence of ethanol by diluting the cephalosporin C solution to certain extent . The dilution method had good precision, reproduction and recovery, which provided a basis for the accurate determination of cephalosporin C in industrial production. |
| |
| Keywords: | cephalosporin C HPLC organic solvent ethanol |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
