| 别藻蓝蛋白藻蓝胆素发色团分子构象研究 |
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| 引用本文： | 夏安东,朱晋昌,蒋丽金,张新夷,孔祥贵.别藻蓝蛋白藻蓝胆素发色团分子构象研究[J].生物化学与生物物理进展,1993,20(2):112-115. |
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| 作者姓名： | 夏安东 朱晋昌 蒋丽金 张新夷 孔祥贵 |
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| 作者单位： | 中国科学院感光化学所;中国科学院感光化学所;中国科学院感光化学所;中国科学院长春物理所激发态过程开放实验室;中国科学院长春物理所激发态过程开放实验室 |
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| 基金项目： | “八五”重大项目和长春物理所激发态过程研究开放实验室资助项目. |
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| 摘 要： | 主要研究了蓝绿藻污棕席藻(Phormidium luridum)别藻蓝蛋白在不同 pH值条件下的吸收光谱和共振拉曼光谱.发现低聚化的结果导致了三聚体别藻蓝蛋白 650nm 特征吸收峰的消失和一些共振拉曼带强度和位置的移动.结果表明在低 pH 值作用下的低聚化的别藻蓝蛋白中藻蓝胆素发色团分子的构象和自由胆素分子类似,比三聚体的别藻蓝蛋白的发色团分子更趋于卷曲,折叠的构象态.而三聚体的别藻蓝蛋白,主要的拉曼带 1645cm-1是其发色团分子构象处于更线性延展的标志,其光谱行为和吸收光谱 Avis/Auv所表征的发色团分子构象的结果相一致.
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| 关 键 词： | 别藻蓝蛋白 共振拉曼光谱 |
| 收稿时间： | 1991/12/11 0:00:00 |
| 修稿时间： | 1992/3/30 0:00:00 |
A Study of Phycocyanobilin Chromophore Conformations in Allophycocyanin |
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| Xia Andong,Zhu Jinchang,Jiang Lijin,Zhang Xinyi and Kong Xianggui.A Study of Phycocyanobilin Chromophore Conformations in Allophycocyanin[J].Progress In Biochemistry and Biophysics,1993,20(2):112-115. |
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| Authors: | Xia Andong Zhu Jinchang Jiang Lijin Zhang Xinyi and Kong Xianggui |
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| Institution: | Institute of Photographic Chemistry, Academia Sinica;Institute of Photographic Chemistry, Academia Sinica;Institute of Photographic Chemistry, Academia Sinica;Laboratory of Excited State Processes, Changchun Institute of Physics;Laboratory of Excited State Processes, Changchun Institute of Physics |
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| Abstract: | The UV absorption and resonance Raman spectra were recorded from the trimer allo-phycocyanin and pH-induced monomer allophycocyanin from blue-green alga Phormidium Iuridum.Monomerization mostly results in the disappearance of characteristic absorption peak 650nm,and in the changes in relative intensities and positions of Raman bands of phycocyanobilin chromophores.As a result,in the monomeric allophycocyanin,the chromophores adopt cyclohelical conformations,which close to these of free chromophores in vitro; and in the trimeric allophycocyanin, fully extended conformations of the chromophores can be marked by the main Raman peak around 1645 cm-1, the behaviour of which is in Rood agreement with the Avis/Auv, absorption ratio. |
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| Keywords: | allophycocyanin resonance Raman spectrum |
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