蜜环菌菌索分化的差异蛋白质组学研究

菌索是蜜环菌与宿主互作的组织结构,蜜环菌菌丝形成菌索的分子机制尚不清楚。本研究采用SWATH-MS^ALL非标记定量蛋白质组学技术,首次对Armillaria mellea菌丝形成菌索过程的蛋白质组学进行了系统研究。在蜜环菌菌丝和菌索中共鉴定蛋白1 724个（global FDR 1%）,定量蛋白1 179个。与菌丝相比,蜜环菌菌索差异表达蛋白640个（上调表达256个,下调表达384个）。差异表达蛋白GO注释结果表明,蜜环菌菌索分化的生物学过程较复杂,差异蛋白参与的主要生物学过程包括有机含氮化合物代谢、有机物生物合成、小分子代谢、氧化还原反应等。分子功能富集结果提示,电子转运活性的富集因子最高,而氧化还原反应的富集P值最显著,这与KEGG注释的氧化磷酸化代谢活跃的结果一致。进一步的推测分析表明,蜜环菌菌丝形成菌索可能是受到氧化应激所致,菌索的形成可能促使诸如硫氧还蛋白样等毒力蛋白和富马酸的合成和释放增多,抑制宿主免疫而有利于蜜环菌侵染和定植于宿主。因此,对差异蛋白质组的进一步研究和深入解析不仅有利于揭示蜜环菌菌索形成的蛋白分子机制,同时也具有较强的理论和现实意义。

Differential proteome of Armillaria mellea in association with fungal rhizomorph differentiation

The mechanism of rhizomorph differentiation from the mycelia of Armillariaspp. is still unclear. The rhizomorph is the essential tissue in the interaction between Armillariaspp. and hosts. In this study, SWATH MS^ALL label-free quantitative proteomics was applied to uncover the mechanism of rhizomorph formation. In total, 1 724 proteins (global FDR 1%) were identified from Armillaria melleahypha (AH) and rhizomorphs (AR) based on data dependent acquisition (DDA) model and 1 179 proteins were quantified based on SWATH (sequential window acquisition of all theoretical fragment ions) label-free model. Compared with AH, 640 proteins differentially expressed at AR, of which 256 were up-regulated and 384 down-expressed. GO analytic results indicated that the differentiation of rhizomorph from hypha was complex, and the main biological processes (BP) involved in differentially expressed proteins (DEPs) included the metabolisms of organonitrogen compound, organic substance biosynthetic process, metabolic process, oxidation-reduction process, small molecule metabolic process etc. The results of molecular function (MF) enrichment suggested that the enrichment factor of electron transport activity was the highest, and the enrichmentP value of oxidation-reduction reaction was the most significant, being consistent with the fact of activated oxidative phosphorylation metabolism annotated by KEGG. Further analysis showed that the differentiation of A.mellea rhizomorph may be induced by oxidative stress, and this promoted the synthesis and release of fumaric acid and virulence proteins such as thioredoxin-like protein to inhibit the immunologic system of hosts, resulting in the easer infection and colonization byArmillariaspp.