| Immunolocalization of Arabinogalactan Proteins and Pectins in Floral Buds of Cucumber （Cucumis sativus L.） During Sex Determination |
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| 作者姓名： | Yi-BenPENG ChengZOU Hua-QinGONG Shu-NongBAI Zhi-HongXU Yi-QinLI |
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| 作者单位： | [1]PekingUniversity-YaleJointResearchCenterofAgriculturalandPlantMolecularBiology,CollegeofLifeSciences,PekingUniversity,Beijing100871,China [2]ProteinScienceLaboratoryoftheMinistryofEducation,SchoolofLifeSciencesandEngineering,TsinghuaUniversity,Beijing100084,China |
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| 摘 要： | Arabinogalactan proteins (AGPs) and pectins were detected in the floral buds of cucumber(Cucumis sativus L.) during its sex determination using the following monoclonal antibodies: MAC 207(recognizes AGP epitopes); JIM 8 (recognizes a subset ofAGP epitopes); and JIM 5 and JIM 7 (epitopes of pectins esterified to various degrees). In the stem apex meristem (SAM) of the cucumber, epitopes of MAC 207, JIM 7, and JIM 5 were localized in the cells from second to third peripheral layers when the sex organ primodium began to differentiate; epitopes of MAC 207 and JIM 5 were also detected in the ragged edge cells. A very dense labeling signal with MAC 207 was observed in the carpel and pistil primodium. The AGP epitopes recognized by JIM 8 were localized in the anther of the male flower and the anther-like portion of the stagnant stamen of the female flower. This suggests that the AGPs and pectins in the SAM of the cucumber are closely associated with the differentiation of the SAM, from meristematic cells to floral primodium. The subset of AGPs recognized by JIM 8 may play an important role in stamen formation.
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| 关 键 词： | 黄瓜 花芽分化 性别决定 免疫定位作用 阿拉伯半乳聚糖蛋白质 果胶 |
Immunolocalization of Arabinogalactan Proteins and Pectins in Floral Buds of Cucumber (Cucumis sativus L.) During Sex Determination |
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| Authors: | Yi-Ben PENG Cheng ZOU Hua-Qin GONG Shu-Nong BAI Zhi-Hong XU Yi-Qin LI |
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| Institution: | Peking University-Yale Joint Research Center of Agricultural and Plant Molecular Biology, College of Life Sciences, Peking University;, Beijing 100871, China; Protein Science Laboratory of the Ministry of Education, School of Life Sciences and Engineering, Tsinghua University;, Beijing 100084, China |
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| Abstract: | Abstract: Arabinogalactan proteins (AGPs) and pectins were detected in the floral buds of cucumber ( Cucumis sativus L.) during its sex determination using the following monoclonal antibodies: MAC 207 (recognizes AGP epitopes); JIM 8 (recognizes a subset of AGP epitopes); and JIM 5 and JIM 7 (epitopes of pectins esterified to various degrees). In the stem apex meristem (SAM) of the cucumber, epitopes of MAC 207, JIM 7, and JIM 5 were localized in the cells from second to third peripheral layers when the sex organ primodium began to differentiate; epitopes of MAC 207 and JIM 5 were also detected in the ragged edge cells. A very dense labeling signal with MAC 207 was observed in the carpel and pistil primodium. The AGP epitopes recognized by JIM 8 were localized in the anther of the male flower and the anther-like portion of the stagnant stamen of the female flower. This suggests that the AGPs and pectins in the SAM of the cucumber are closely associated with the differentiation of the SAM, from meristematic cells to floral primodium. The subset of AGPs recognized by JIM 8 may play an important role in stamen formation.
(Managing editor: Wei WANG) |
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| Keywords: | arabinogalactan proteins cucumber (Cucumis sativus L ) floral buds immunolocalization pectins |
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