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The use of an oligonucleotide that hybridizes to DNA sequences interspersed in the genome of members of the genusPhytophthora as an adjunct to morphological criteria in species identification |
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| Authors: | David Scott Clarence Clark Andrea Fyffe Michon Walker Paul Tooley John Maas Ken Deahl |
| Affiliation: | 1. Department of Biology, Morehouse College, 830 Westview Drive, 30314, Atlanta, GA 2. Center for Plant Biotechnology Research, Tuskegee University, Tuskegee, AL 3. USDA-ARS, Ft. Detrick, MD, USA 4. Fruit Laboratory, USA 5. Vegetable Laboratory, USDA-ARS, Beltsville, MD |
| Abstract: | An oligonucleotide (primer), designed from a conserved region of the multi-allelicb locus of the basidiomycete fungusUstilago maydis, generated reproducible PCR fingerprints inPhytophthora species. The primer hybridized in a species-specific manner to nucleotide sequences interspersed in the genome of the closely related members ofPhytophthora taxonomic group IV. We recommend the use of this PCR procedure as an alternative method for resolving the close taxonomic affinity of some members of the genusPhytophthora. |
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