Immunocytochemical localization of methyl-coenzyme M reductase in Methanobacterium thermoautotrophicum |
| |
| Authors: | H C Aldrich D B Beimborn M Bokranz P Schönheit |
| |
| Institution: | (1) Fachbereich Biologie-Mikrobiologie, Philipps-Universität Marburg, Karl-von-Frisch-Strasse, D-3550 Marburg, Federal Republic of Germany;(2) Present address: Department of Microbiology and Cell Science, University of Florida, 32611 Gainesville, FL, USA |
| |
| Abstract: | Cells of Methanobacterium thermoautotrophicum were fixed with glutaraldehyde, sectioned and labeled with antibodies against the  subunit of component C (=methyl-CoM reductase) of methyl-CoM reductase system and with colloidal gold-labeled protein A. It was found that the gold particles were located predominantly in the vicinity of the cytoplasmic membrane, when the cells were grown under conditions where methyl-CoM reductase was not overproduced. This finding confirms the recent data obtained with Methanococcus voltae showing via the same immunocytochemical localization technique that in this organism methyl-CoM reductase is membrane associated. |
| |
| Keywords: | Methanobacterium thermoautotrophicum Methyl-CoM reductase Immunocytochemistry Colloidal gold Energy conservation |
| 本文献已被 SpringerLink 等数据库收录！ |
