|
|||||
|
|
Cryopreservation of shoot-tips by droplet vitrification applicable to all taro (Colocasia esculenta var. esculenta) accessions |
|
| Authors: | Rajnesh Sant Bart Panis Mary Taylor Anand Tyagi |
| Affiliation: | (1) School of Life Sciences, Queensland University of Technology, GPO Box 2434, Brisbane, QLD, 4001, Australia;(2) Laboratory of Tropical Crop Improvement, Division of Crop Biotechnics, K.U. Leuven, Kasteelpark Arenberg 13, B-3001 Leuven, Belgium;(3) Secretariat of the Pacific Community, Private Mail Bag, Suva, Fiji;(4) Department of Biology, The University of the South Pacific, P.O. Box 1168, Suva, Fiji |
| Abstract: | The application of the droplet vitrification cryopreservation technique to taro accessions from a range of Asia Pacific countries is presented. The optimum protocol involves excision of about 0.8 mm shoot-tips from in vitro plants, 20–40 min PVS2 exposure at 0°C followed by rapid plunge into liquid nitrogen. Thawing was done at room temperature (25°C) and shoot-tips inoculated on MS medium with 0.1 M sucrose regenerated into plantlets 4–6 weeks later. This new droplet vitrification protocol improved the mean post-thaw regeneration rates to 73–100% from 21–30% obtained with the previous cryo-vial vitrification protocol. |
| Keywords: | Taro Cryopreservation Shoot-tips Droplet vitrification |
| 本文献已被 SpringerLink 等数据库收录！ | |