大鼠产前手机辐射对子代小脑白质的影响

目的:探讨大鼠产前850～1 900 MHz手机辐射对成年子代小脑白质的影响。方法:孕鼠随机分为短时产前手机辐射组、长时产前手机辐射组和对照组,短时和长时辐射组于孕期第1～17日分别进行每天6 h和24 h手机辐射,各组雄性子代大鼠(n＝8)于3月龄取小脑组织,进行苏木精-伊红(HE)染色观察小脑皮质细胞形态,免疫组化和Western blot检测髓鞘碱性蛋白(MBP)、神经微丝-L(NF-L)和胶质纤维酸性蛋白(GFAP)表达。结果:与对照组比较,短时程和长时程产前手机辐射组子代大鼠小脑浦肯野细胞出现形态学改变;与对照组比较,长时程辐射组MBP、NF-L表达明显减少(P均＜0.05),而GFAP表达明显增多(P＜0.05);与短时程辐射组比较,长时程组子代大鼠小脑MBP、NF-L表达明显减少(P均＜0.05),而GFAP表达明显增多(P＜0.05)。结论:产前手机辐射会导致雄性子代大鼠小脑髓鞘和轴突的损害,以及星形胶质细胞的活化,且这种改变与产前手机辐射的时程相关。

The effects of prenatal radiation of mobile phones on white matter in cerebellum of rat offspring

Objective:To evaluate the effects of prenatal radiation of 850~1900 MHz mobile phone on white matter in cerebellum of adult rat offspring.Methods:Pregnant rats were randomly divided into short term maternal radiation group,long term maternal radiation group and control group.Rats in short term and long term maternal radiation group were exposed to 6 h/d and 24 h/d mobile phone radiation during 1-17 days of pregnancy,respectively.The cerebellums of offspring rats at the age of 3 month(n=8)were taken.Cell morphology in cerebellum was studied by hematoxylin-eosin(HE)staining.The expressions of myelin basic protein(MBP),neurofilament-L(NF-L)and glial fibrillary acidic protein(GFAP)in cerebellum of rat offspring were detected by immunohistochemistry and Western blot.Results:Compared to control group,the morphological changes of purkinje cells in cerebellum were obvious in rat offspring of short term and long term maternal radiation group.Compared to control group,decreased MBP and NF-L expressions and increased GFAP expression were observed in long term maternal radiation group(all P<0.05).Compared to short term radiation group,the expressions of MBP and NF-L were down-regulated(all P<0.05)and the expression of GFAP was up-regulated(P<0.05)in long term radiation group.Conclusion:Prenatal mobile phone radiation might lead to the damage of myelin and axon with activity of astrocytes in cerebellum of male rat offspring,which is related to the extent of radiation.