| 从玉米胚中大量制备植物钙调素 |
| |
| 引用本文： | 龚明,杨中汉.从玉米胚中大量制备植物钙调素[J].云南植物研究,1996,18(1):93-98. |
| |
| 作者姓名： | 龚明 杨中汉 |
| |
| 作者单位： | 云南师范大学生命科学系，北京大学生命科学学院 |
| |
| 基金项目： | 国家自然科学基金,云南省科委配套基金,云南省教委科研基金 |
| |
| 摘 要： | 从每公斤萌发２４ｈ的玉米胚丙酮粉中可提纯得６３ｍｇ的钙调素（ＣａＭ），这是目前从每公斤植物材料中所提纯得ＣａＭ的最高记录。对其理化性质的研究表明，玉米胚ＣａＭ 有较高的生物学活性，能较好地激活磷酸二酯酶，其紫色吸收光谱，ＳＤＳ－ＰＡＧＥ电泳行为及氨基酸组成与其它的植物ＣａＭ相似。上述结果表明玉米胚是１个提取植物ＣａＭ相似。上述结果表明玉米胚是１个提取植物ＣａＭ的适宜材料。
|
| 关 键 词： | 玉米 胚 调钙蛋白 |
MASS PREPARATION OF PLANT CALMODULIN FROM MAIZE GERM |
| |
| GONG Ming,DU Chao-Kun,YANG Zhong-Han,CAO Zong-Xun,.MASS PREPARATION OF PLANT CALMODULIN FROM MAIZE GERM[J].Acta Botanica Yunnanica,1996,18(1):93-98. |
| |
| Authors: | GONG Ming DU Chao-Kun YANG Zhong-Han CAO Zong-Xun |
| |
| Abstract: | mg calmodulin was extracted and purified from 1 kg acetone powder of maize germ germinated for 24 h, which is the highest record for the quantity of calmoulin purified from 1 kg plant materials. Our results showed that the calmodulin from maize germ had higher biological activity, could activate cAMP phosphodiesterase within a good range, and its UV absorption spectrum, electrophoretic mobility on SDS-PAGE in the presence or absence of Ca2 and amino acid composition was similar to those calmodulins from other plant materials. The above-mentioned results indicated that maize germ is a suitable material for the extraction and purification of plant calmodulin. |
| |
| Keywords: | Calmodulin Maize Germ |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
